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. 2010 Sep 1;299(6):F1258–F1269. doi: 10.1152/ajprenal.00374.2010

Fig. 4.

Fig. 4.

Confocal microscopy examining Kv1.3 channel and CD3 or ED-1 in mononuclear cell suspensions. A: double-immunofluorescent staining for CD3 (green) and Kv1.3 (red) with nuclear staining (Hoechst 33342: blue) on isolated mononuclear cells. Merged image is shown in far right lane. Large CD3+ T cells (arrowhead) and small CD3+ T cells (arrow) are visible (original magnification, ×200). Top right inset: high-magnification view of a large CD3+ T cell (original magnification, ×600). Kv1.3 was expressed on most of the CD3+ T cells. B: double-immunofluorescent staining for ED-1 (green) and Kv1.3 (red) with nuclear staining (Hoechst 33342: blue) on isolated mononuclear cells. Merged image is shown in the rightmost lane. Kv1.3 was expressed on some (*), but not all, of the ED-1+ macrophages (original magnification, ×200).