Table 1.

Antibodies used in this study

Specificity	Clone	Labeling	Origin	Pretreatment for IHC
CD3	1F4, 1F4, 4.18	Unlabeled, Alexa−488, PE	c, c, a	Autoclave
CD4	W3/25	biotin	b
CD8	OX8	PE-Cy5	a
CD11b/c	OX42	PE	c, a
CD45RC	OX22	FITC, PE	c, c	Proteinase K
CCR7	E75	Unlabeled	e	Proteinase K
CD62L	OX85	PE, biotin	c, c
CD68	ED1	PE, unlabeled, Alexa-488	c, c, c	Proteinase K
CD161a	3.2.3	PE	c
Kv1.3	L23/27, polyclonal	Unlabeled, FITC	d, f	Autoclave
αβTCR	R7/3	PE, unlabeled	a, a
γδTCR	V65	PE, unlabeled	a, a
Biotin		PE-Cy5, PE	a, a

IHC, immunohistochemistry; proteinase K, incubated with proteinase K (Dako) for 8 min; autoclave, boiled in citrated buffer (pH 6) via autoclave (121°C) for 15 min; PE, phycoerythin. ^aBD bioscience; ^bBiolegend; ^cSerotec; ^dAlomone Lab; ^eEPTMICS; ^fNeuroMab.