Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Oct 6;299(6):F1507–F1514. doi: 10.1152/ajprenal.00183.2010

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 the American Physiological Society

PMC Copyright notice

Fig. 1. — Isolation of cortical collecting duct (CCD) cells by flow cytometry. Top left: unstained cell fluorescence dot plot. Top right: Dolichos biflorus agglutinin (DBA)-stained cells showed a shift in fluorescence that is separate from unstained cells, and FACSAria software was gated to select the DBA-positive cells. Bottom left: brightfield image of mouse telomerase reverse transcriptase (mTERT)-CCD cells grown on glass at passage 25 (×20 objective with ×10 camera zoom). Bottom right: brightfield image of mTERT cells grown on Snapwell membranes at passage 30 (×20 objective with ×10 camera zoom) showing typical epithelial cell morphology.