Fig. 2.

Quantatative (q)RT-PCR of TERT mRNA from mTERT-CCD cells and a collecting duct cell line expressing normal Tg737 (BAP2) shows a 32-fold higher expression of TERT mRNA in the mTERT-immortalized cells. Error bars represent SD; n = 6. Real-time quantitative telomeric repeat amplification protocol (RTQ-TRAP) results confirm there is 8.5-fold higher telomerase activity in mTERT-CCD cells compared with BAP2 cells. Results are calculated using differences in cycle threshold between the 2 cell lines. *P = 0.002.