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Overexpression of dynamin decreases UT-A1 cell surface accumulation. UT-A1-HEK293 cells were transfected with pEGFP, pEGFP-Dynamin, or pEGFP-K44A for 48 h. Cells were processed for biotinylation. Total cell lysate and biotinylated samples were immunoblotted with UT-A1 or GFP antibodies.
