Fig. 5.

Overexpression of dynamin, caveolin, and μ2 reduces UT-A1 activity. A: oocytes were injected with cRNA encoding UT-A1 (2 ng/cell) alone or together with dynamin, caveolin, or μ2 (5 ng/cell) for 3 days. Urea transport activity was measured by [¹⁴C] urea flux (n = 6 oocytes/time point). B: UT-A1 expression in total cell lysates or biotinylated samples was analyzed by immunoblotting with UT-A1 antibody. The results are representative of 3 separate experiments. *P < 0.05, **P < 0.01.