Fig. 1.

Mammalian target of rapamycin (mTOR) expression is elevated in patients with heart failure. A: mTOR protein levels. Top, representative Western blots showing mTOR protein levels in extracts of human heart tissue from donor control and heart failure patients. GAPDH was used as a loading control. Bottom, densitometric quantification of mTOR protein expression. Values are means ± SE. Data are expressed as the degree of change (fold change) over the mean value in donor hearts. B: mTOR mRNA levels. mTOR mRNA was measured in extracts of donor and patient hearts by quantitative RT-PCR (QRT-PCR). Values are means = SE; n = 5 for each group.