Fig. 7.

IL-1β induced nuclear-to-cytosolic translocation of HuR and increased the binding of HuR to RGS4 coding region in rabbit colonic smooth muscle cells. A: representative immunofluorescent micrographs of HuR nuclear-to-cytosolic translocation in cultured cells after IL-1β treatment for 30 min. B: typical example of HuR fluorescent image analysis and quantitative data after Il-1β treatment for 30 min. **P < 0.01, statistically significant decrease by Student's t-test compared with control. C: dynamic changes in HuR protein expression in nuclear and cytosolic fractions determined by Western blot analysis. D: biotin-RNA pull-down assay showing that both UTR and coding sequence (cds) of rabbit RGS4 specifically bind to HuR and IL-1β stimulation increases binding activity of both cytosolic and nuclear HuR to RGS4 coding region.