Fig. 3.
Free zinc levels are increased in human umbilical vein endothelial cells (HUVECs) exposed to shear stress. HUVECs subjected to 24-h shear stress treatment were stained with FluoZin-3-AM. A: fluorescent images were converted to pseudocolor to show fluorescence intensity (scale bar is 50 μm). B: representative flow cytometry profile (red, static; blue, reversing flow; green, 1 dyn/cm2; black, 15 dyn/cm2). C: histograms showing mean fluorescence (n = 7; *P < 0.01, compared with static culture; ‡P < 0.01, between indicated conditions). Free zinc is highest under 15 dyn/cm2, attenuated under 1 dyn/cm2 and reversing flow, and lowest under static conditions.