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. 2010 Oct 6;299(6):C1524–C1529. doi: 10.1152/ajpcell.00168.2010

Fig. 4.

Fig. 4.

Inhibition of phosphatidylinositol 3-kinase (PI3K) annuls the effect of T3 or TR-α1 overexpression on ERK phosphorylation. Western blot shows analysis of ERK phosphorylation in cells cultured with T3-free serum only (Ctr), PMA (300 nM) added, PMA plus LY294002 (LY, 50 μM), PMA plus 1 nM T3 and adenovirus expressing TR-α1, and PMA plus 1 nM T3 and adenovirus expressing TR-α1 and LY. Inhibition of PI3K blocked the inhibitory effect of thyroid hormone action on ERK phosphorylation. Values are means ± SE. *P < 0.05 vs. Ctr, PMA + LY, PMA + T3 + TR-α +LY; **P < 0.05 vs. Ctr, PMA, PMA + T3 + TR-α + LY; *P < 0.05 vs. PMA + LY, PMA + T3 + TR-α + LY; &P < 0.05 vs. Ctr, PMA, PMA + LY, PMA + T3 + TR-α; n = 6 from 3 experiments.