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. 2010 Dec 21;8(12):e1000565. doi: 10.1371/journal.pbio.1000565

Figure 5. Forced expression of Prox1 in mouse NPCs suppresses progenitor identity, inhibits astrocyte differentiation, and induces neurogenesis.

Figure 5

(A–D) Relative luciferase activities (A and C) and mRNA levels (B and D) were measured in NPCs transfected with various constructs as indicated. (E–G) Double GFP or Flag/BrdU immunostainings of NPCs in the presence of GFs and electroporated with GFP (E) or Prox1 (F) expression vectors. In all panels of this figure, expression of transgenes was detected with anti-GFP or anti-Flag antibodies, respectively. Quantification of BrdU index is shown in (G). p<0.001. (H–J) Double GFP or Flag/Nestin immunostainings of NPCs cultured in the presence of GFs and electroporated with GFP (H) or Prox1 (I) expression vectors. Quantification of Nestin index is shown in (J) p<0.001. (K–M) Double GFP or Flag/GFAP immunostainings of NPCs cultured in the absence of GFs and electroporated with GFP (K) or Prox1 (L) expression vectors. Quantification of GFAP index is shown in (M) p<0.001. (N–P) Double GFP or Flag/βIII-tubulin immunostainings of NPCs cultured in the absence of GFs and electroporated with GFP (N) or Prox1 (O) expression vectors. Quantification of βIII-tubulin index is shown in (P) p<0.01. For all panels scale bar: 50 µm.