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. 2010 Dec 21;8(12):e1000565. doi: 10.1371/journal.pbio.1000565

Figure 7. Active Notch1 signaling suppresses Prox1 gene expression.

Figure 7

(A–B) RT-PCR analysis of NPCs cultured in the presence or absence of DAPT, for Hes1, Hes5, Gapdh (A), and Prox1 (B) genes, as indicated. For Prox1 mRNA, p<0.01, n = 3. (C–H') Double GFP/Prox1 immunostainings and in situ hybridizations for Prox1 in consecutive sections, as indicated, 48 h a.e. with NICD/GFP (C–E') or GFP alone (F–H'). (D'), (E'), (G'), and (H') micrographs are larger magnifications of the electroporated area in (D), (E), (G), and (H), respectively. Scale bar: 50 µm.