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. 2010 Dec 20;5(12):e15636. doi: 10.1371/journal.pone.0015636

Figure 6. Expression of Praf2 induces apoptotic cell death.

Figure 6

(A) FACS analysis of HeLa cells transfected with the indicated expression vectors and stained with Propidium Iodide (PI). Percentages of PI positive cells are indicated on the top-left of each graph. (B) Aliquots of the HeLa cells transfected as in (A) were analysed by immunoblot for the appearance of the cleaved form of PARP. Expression levels of Praf2, Bcl-xL and Bcl-xLΔTM are also indicated. (C) Analysis of GFP-Bax localisation in U2OS cells transfected with empty vector (vec) or HA-tagged Praf2. The photographs are representative of the two main GFP-Bax localisations observed in the samples: diffuse cytosolic and aggregated. Above the photographs are indicated the % of GFP positive cells with an aggregated localisation after transfecting cells with empty vector or HA-tagged Praf2 in two independent experiments.