TABLE V. 3-Hydroxy-3-Methylglutaryl-CoA (Hmg-CoA) Reductase Activity in Hepatocytes from Normal and Homozygous Familial Hypercholesterolemia Patients.

Hepatocytes (27–60 μg cellular protein/well) were incubated in serum-free medium for 48 h followed by another 24 h in serum-free medium or serum-free medium with 200 μg LDL protein/ml. Cells were harvested and the ability to generate mevalonate was determined [27].

	HMG-CoA reductase activity (pmol/mg protein/min)
	serum-free medium	serum-free medium + LDL
Normal hepatocytes (n = 3)	3.1 ± 0.6	0.6 ± 0.2
Patient 1 hepatocytes	4.8	5.6
Patient 2 hepatocytes	6.4	6.2