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IR-ELDI-MS limit of detection for peptides and proteins. ELDI mass spectra from (A) 0.5 μL of 0.5 μM (250 fmol) of bradykinin with S/N 20, (B) 0.5 μL of 1 μM (500 fmol) of carbonic anhydrase with S/N 3, and (C) 1 μL of 0.1 μM (100 fmol) of ubiquitin. (A) and (B) were acquired using an LTQ linear ion trap, while (C) was acquired using anLTQ-OrbiTrap.
