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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: J Comp Neurol. 2010 Sep 15;518(18):3771–3784. doi: 10.1002/cne.22423

Figure 3.

Figure 3

Staining, antigenic, and morphologic characteristics of dystrophic axons. Axonal swellings stained pink with H&E (panel A, red nucleus) and PAS (panel B, rostral cerebellar peduncle shown, stained after diastase digestion), and grey to black with Holme's silver stain (panel C, arrowhead indicates a spheroid in the rostral cerebellar peduncle). Immunohistochemical staining was either strongly positive or negative (arrowheads) when the primary antibody (SMI 312) was directed at phosphorylated neurofilaments (panel D). Panels E and F show serial cross-sections of the ventral medial funiculus of cervical spinal cord segment 6 immunostained for neuron-specific enolase (NSE) and GFAP, respectively. Dystrophic axons throughout the CNS uniformly stained positive for NSE but never for GFAP (see also fig. 4). Bars in panels A-F = 20 μm. Transmission electron microscopy of dystrophic axons in the red nucleus (panel G) and medullary tegmentum (panel H) showed accumulations of membrane-bound vacuoles containing variably electron-dense amorphous material or organelles in various stages of degeneration (bars in panels G and H = 1 μm.