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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: J Comp Neurol. 2010 Sep 15;518(18):3771–3784. doi: 10.1002/cne.22423

Table 1. Primary Antibodies.

Antigen Immunogen Manufacturer, species, type, cat. # Dilution
glial fibrillary acidic protein Purified bovine spinal cord GFAP Dako, Carpinteria, CA, rabbit polyclonal #Z0334, lot 00015316 1:500
neuron-specific enolase Purified bovine brain NSE, γγ isoenzyme Dako, Carpinteria, CA, rabbit polyclonal, #A587, lot 0045 1:300
calbindin D-28k Recombinant mouse 28 kDa calbindin Chemicon International, Inc., Temecula, CA rabbit polyclonal #AB1778 1:2000
Phosphorylated heavy and medium neurofilament proteins [SMI- 312] Homogenized Fisher 344 rat hypothalamus Abcam, Inc., Cambridge, MA, mouse monoclonal cocktail, #ab24574, lot 616423 1:1000
activated caspase-3 KLH-linked synthetic peptide CRGTELDCGIETD adjacent to Asp175 in human caspase 3 Cell Signaling Technology, Beverly, MA, rabbit polyclonal #9661, affinity purified on immunogen column 1:100