glial fibrillary acidic protein |
Purified bovine spinal cord GFAP |
Dako, Carpinteria, CA, rabbit polyclonal #Z0334, lot 00015316 |
1:500 |
neuron-specific enolase |
Purified bovine brain NSE, γγ isoenzyme |
Dako, Carpinteria, CA, rabbit polyclonal, #A587, lot 0045 |
1:300 |
calbindin D-28k |
Recombinant mouse 28 kDa calbindin |
Chemicon International, Inc., Temecula, CA rabbit polyclonal #AB1778 |
1:2000 |
Phosphorylated heavy and medium neurofilament proteins [SMI- 312] |
Homogenized Fisher 344 rat hypothalamus |
Abcam, Inc., Cambridge, MA, mouse monoclonal cocktail, #ab24574, lot 616423 |
1:1000 |
activated caspase-3 |
KLH-linked synthetic peptide CRGTELDCGIETD adjacent to Asp175 in human caspase 3 |
Cell Signaling Technology, Beverly, MA, rabbit polyclonal #9661, affinity purified on immunogen column |
1:100 |