Table 1.
Pattern of expression of the sequences affected by cold stress and heat shock
Pattern of expression | During thermal stress (T = 0 h) | After recovery (T = 3 h) | |||||
---|---|---|---|---|---|---|---|
SignificantaN = 2,987 | Significantly affected ≥twofold | High certaintyb | SignificantaN = 2,632 | Significantly affected ≥twofold | High certaintyb | ||
Affected only by cold | Total | 2,357 | 381 | 323 | 1,310 | 162 | 98 |
Increased | 1,222 | 138 | 114 | 748 | 73 | 51 | |
Decreased | 1,135 | 243 | 209 | 562 | 89 | 47 | |
Affected only by heat | Total | 389 | 52 | 24 | 975 | 195 | 128 |
Increased | 144 | 30 | 14 | 362 | 126 | 87 | |
Decreased | 245 | 22 | 10 | 613 | 69 | 41 | |
Similarly affected by cold and heat | Total | 160 | 12 | 296 | 13 | ||
Increased | 21 | 2 | 50 | 8 | |||
Decreased | 139 | 10 | 246 | 5 | |||
Oppositely affected by cold and heatc | Total | 81 | 3 | 3 | 51 | 0 | 0 |
Cold up–heat down | 54 | 0 | 0 | 48 | 0 | 0 | |
Cold down–heat up | 27 | 3 | 3 | 3 | 0 | 0 | |
Cold totals | Significantly different from controls | 2,598 | 409d | 1657 | 192d | ||
Increased | 1,297 | 143d | 846 | 83d | |||
Decreased | 1,301 | 266d | 811 | 109d | |||
Heat totals | Significantly different from controls | 630 | 71d | 1322 | 231d | ||
Increased | 192 | 35d | 415 | 146d | |||
Decreased | 438 | 36d | 907 | 85d |
aImplies that a significant difference was found both by two-way and one-way ANOVA and that post-hoc comparisons showed a significant difference between control and one or both of the thermal stress conditions
bImplies that there was also a significant difference between responses to heat and cold in the post-hoc comparisons
cAll of these showed, in the post-hoc analysis, a significant difference between control conditions and each of the thermal conditions tested, as well as a significant difference between heat and cold
dComputed without regard to the magnitude of the effect of the opposite thermal stimulus and therefore can add up to more than the sum of the previous categories