Fig. 1.

FRT recruits HSF-1 only to the 5′-UTR in the TNF-α promoter. a RAW 264.7 macrophages were warmed at 39.5°C for 90 or 150 min, and cross-linked chromatin was analyzed for recruitment of HSF-1 to the HSE-containing region of the HSPA1A promoter and the TNF-α 5′-UTR by ChIP assay and expressed as enrichment compared with nonspecific IgG control. b Cross-linked chromatin from RAW 264.7 cells warmed to 39.5°C for 90 min was analyzed for recruitment of HSF-1 to overlapping ∼150 bp amplicons comprising the 1.3 kb TNF-α 5′ flanking sequence (see Table 1 for sequences) and expressed as enrichment compared with nonspecific IgG control. Data presented as mean ± SE of five independent experiments. *p < 0.05 compared with untreated controls