Fig. 3.

FRT abrogates recruitment of Sp1 to TNF-α minimal promoter. RAW 264.7 macrophages were prewarmed to 37°C or 39.5°C for 30 min, stimulated with 100 ng/ml LPS and incubated at the same temperature for an additional 60 min. Cross-linked chromatin was analyzed for recruitment of Sp1 (a), ATF2 (b), and Elk-1 (c) to the TNF-α minimal promoter (−208/+32) by ChIP assay and expressed as fold-change vs. unstimulated 37°C cells. Data presented as mean ± SE of five independent experiments. *p < 0.05 compared with untreated controls. †p < 0.05 vs. 37°C cells at the same time point