Fig. 5.

Exposure to FRT fails to alter histone H3 acetylation of the TNF-α promoter. RAW 264.7 macrophages were warmed to 39.5°C for 90 min and analyzed for acetylation of histone-H3 by ChIP on overlapping ∼150 bp regions of the TNF-α proximal promoter. Histone acetylation for each promoter region at 39.5°C was expressed as fold-change compared with 37°C cells (indicated by dashed line). Data presented as mean ± SE of five independent experiments