Table 1.
Primer sequences and amplicon position along the human HSPA1A and TNF-α gene
| Gene | Amplicon distance from start site | Primer sequences |
|---|---|---|
| HSPA1A | −250/−51 | F, AGCACCAGCACTTCCCCACA |
| R, CCGCTGGGCCAATCAGCGAG | ||
| TNF-α | −1,300/−1,175 | F, AGAGAGATGGCGAGAGAATTAGATG |
| R, TGGGTTTCACAGATGGTGCA | ||
| TNF-α | −1,194/−1,063 | F, TGCACCATCTGTGAAACCCA |
| R, TGGACCAACTGAGGCCTCTG | ||
| TNF-α | −1,082/−890 | F, CAGAGGCCTCAGTTGGTCCA |
| R, ACCCTCCAGTGGAGTCACTTCTC | ||
| TNF-α | −912/−763 | F, GAGAAGTGACTCCACTGGAGGGT |
| R, ACTGCGGTACATCAACTCAGACAT | ||
| TNF-α | −786/−662 | F, ATGTCTGAGTTGATGTACCGCAGT |
| R, GAATTCACGGACCTCACAAGC | ||
| TNF-α | −685/−543 | F, AAGGCTTGTGAGGTCCGTGA |
| R, AAGTGGCTGAAGGCAGAGCA | ||
| TNF-α | −586/−468 | F, ATGCACACTTCCCAACTCTAAG |
| R, CTTCTGAAAGCTGGGTGCATAAG | ||
| TNF-α | −490/−356 | F, TTATGCACCCAGCTTTCAGAAG |
| R, TCCTCCAGAGCTAATCATTGTCTG | ||
| TNF-α | −364/−182 | F, TCTGGAGGACAGAGAAGAAATG |
| R, GGTTTGGAAAGTTGGGGACAC | ||
| TNFa | −208/−89 | F, TTCCTTGATGCCTGGGTGTC |
| R, CCACCAGGATTCTGGCAATG | ||
| TNF-α | −89/+32 | F, TTTTCCGAGGGTTGAATGAGA |
| R, TCCTGGCTAGTCCCTTGCTGT | ||
| TNF-α | +4/+152 | F, AGCGAGGACAGCAAGGGA |
| R, TCTTTTCTGGAGGGAGTGTGG |
Primers are listed 5′ to 3′
F forward, R reverse