Fig. 6.

Relative amounts of subcellular CHIP are slightly altered immediately following OGD (a). Total CHIP levels did not change significantly during recovery following 30 and 120 minutes of OGD (b). Equal protein concentrations from whole-cell lysate of primary mixed cultures were probed for CHIP. Equal protein concentrations from subcellular fractions of primary mixed cultures exposed to OGD were probed for CHIP by Western blot (c). No changes are observed in the cytoplasmic fraction with increasing durations of OGD. In the nuclear fraction, a slight increase is observed following 30 min of OGD is sustained, but not otherwise altered following increasing durations of OGD. Elevated levels of nuclear CHIP are maintained during recovery after 30 min of OGD. Equal protein concentrations from subcellular fractions of primary mixed cultures exposed to mild (30 min) and severe (120 min) OGD were probed for CHIP, HSC70, and HSP70 by Western blot