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siRNA Northern blots were hybridized with sense RNA probes for each of the transposons indicated in (A) and (B) in order to detect 25 nt antisense siRNA from each of the sequences tested. AtMu1 is single copy so that autoradiographic exposure was increased substantially. A 22 oligonucleotide size marker is indicated, and the 21 nt miRNA miR-171 was used as a loading control. It is unaffected in the mutants tested.
