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. 2003 Dec 22;1(3):e83. doi: 10.1371/journal.pbio.0000083

Figure 2. Downregulation of HIF2α Is Sufficient to Suppress Tumor Growth by pVHL-Defective Renal Carcinoma Cells.

Figure 2

(A) Parental 786-O cells (VHL[−/−]) and 786-O cells infected with an empty retrovirus (Empty) or retroviruses encoding HIF2α shRNAs (sequence #2 or #3) were grown in the presence of 21% or 1% oxygen and immunoblotted (IB) with the indicated antibodies.

(B) In vitro proliferation of 786-O cells infected with the indicated retroviruses.

(C) Tumor weights approximately 9 wk after subcutaneous implantation of 786-O cells infected with the indicated retroviruses in nude mice. Number of tumors analyzed is shown in parentheses. Error bars = one standard error.

(D) Representative photograph of nude mouse 9 wk after subcutaneous injection of 786-O cells in left (upper) flank and 786-O cells infected with HIF2α shRNA (#3) retrovirus on right (lower) flank.