Figure 5.
ELF4 localizes to the nucleus. Transient transfection assays in onion epidermal cells using EGFP-ELF4 constructs. A and B, Cells expressing the EGFP-ELF4 fusion protein; C and D, cells expressing the EGFP-protein control. GFP florescence (A) and propidium iodide (PI)-stained (B) nuclei show that all of the detectable EGFP-ELF4 fusion protein is nuclear localized. GFP fluorescence (C) and PI-stained (D) nuclei show that the EGFP protein control is distributed throughout the cell, including the cytoplasm and nucleus.