Figure 6.

The Met docking site peptide inhibits in vivo angiogenesis. A cocktail of HGF (50 ng/ml) and heparin (50 U/ml) promoted a hemorrhagic vascularization of the matrigel sponge clearly detectable at 4 days post-implantation. HGF-induced vascularization of the subcutaneously injected matrigel implants was significantly inhibited by the addition of micromolar concentration of Met peptides, as detectable at visual inspection (A) and quantified by measuring hemoglobin (Hb) content of the pellets (B) (***P<0.001). Representative histological specimens that were stained with hematoxylin and eosin show extensive necrotic areas (C (a, b and c)). CD11b/c immunohistochemistry on adjacent sections show abrogation/impairment of cellular infiltrates in the pellets treated with the peptides (C (a′, b′ and c′)) ( × 400 magnification).