Skip to main content
. 2010 Dec 21;16(47):5965–5974. doi: 10.3748/wjg.v16.i47.5965

Figure 1.

Figure 1

Detection of carcinoembryonic antigen, cytokeratin 20 and epidermal growth factor receptor mRNAs in colorectal cancer tissue samples, HT-29 colorectal cancer cells and colorectal cancer patient peripheral blood samples. A: Detection of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) mRNA in pairs of colorectal cancer (CRC) and normal adjacent tissue samples. Lane 0 = molecular weight marker (100 bp); lane 1 = normal tissue; lane 2 = CRC tissue; B: Assessment of the sensitivity of the reverse transcription polymerase chain reaction (RT-PCR) detections for CEA, CK20 and EGFR mRNA. Lane 0 = molecular weight marker (100 bp); lane 1 = 105 HT-29 cells in 3 mL of normal blood; lane 2 = 104 HT-29 cells in 3 mL of normal blood; lane 3 = 103 HT-29 cells in 3 mL of normal blood; lane 4 = 102 HT-29 cells in 3 mL of normal blood; lane 5 = 10 HT-29 cells in 3 mL of normal blood; lane 6 = 1 HT-29 cell in 3 mL of normal blood; lane 7 = normal blood; C: Assessment of the sensitivity of the multiplex RT-PCR detections for CEA (131 bp), CK20 (371 bp) and EGFR (474 bp) mRNA. Lane 0 = molecular weight marker (100 bp); lane 1 = 105 HT-29 cells in 3 mL of normal blood; lane 2 = 104 HT-29 cells in 3 mL of normal blood; lane 3 = 103 HT-29 cells in 3 mL of normal blood; lane 4 = 102 HT-29 cells in 3 mL of normal blood; lane 5 = 10 HT-29 cells in 3 mL of normal blood; lane 6 = 1 HT-29 cell in 3 mL of normal blood; lane 7 = normal blood; D: An example of multiplex RT-PCR-based detection pattern of tumor marker transcripts in the peripheral blood samples of CRC patients. Left: lane 0 = molecular weight marker (100 bp); lane 1 = patient sample positive for CEA (131 bp) and CK20 (371 bp) mRNA; lane 2 = patient sample positive for CEA (131 bp) mRNA; lane 3 = patient sample positive for CEA (131 bp), CK20 (371 bp) and EGFR (474 bp) mRNA; lane 4 = patient sample negative for all markers. Right: lane 0 = molecular weight marker (100 bp); lanes 1-4 = amplification of β-actin (172 bp) cDNA of the patient samples presented in the left side of this panel.