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Time series of mitochondria after addition of nocodazole to depolymerize microtubules. Experiment was performed in a fibroblast line stably expressing mitoRFP. Time lapse images were acquired following Basic Protocol 1 and Support Protocol 1. The numbers represent time (in minutes) after addition of nocodazole. 543nm HeNe laser was used. The raw image has been converted into gray scale. Images were acquired by a 63× objective in zoom 2. Pinhole size was 2.5 airy units.
