Figure 3.

Fluorescence Recovery after Photobleaching on single mitochondria. Experiment was performed in a fibroblast line stably expressing mitoRFP following Basic Protocol 2. The time scale of recovery is in milliseconds. The arrow points to the bleached zone. Another mitochondria in the field of view gives an assessment of overall bleaching during the recovery period. High speed laser scanning confocal microscope (Zeiss LSM5 Duo) was used in A while a similar but slower microscope (Zeiss 510) was used in B. Prebleach and postbleach images depict mitochondria before and after the single bleaching pulse respectively. 543nm HeNe laser line was used for imaging and other imaging settings used is mentioned in Table 2.