Figure 5.
Analysis of connectivity by FRAP on mitochondrial population. Images of cell A/B of Figure 4 was analyzed according to (Goodwin & Kenworthy 2005). Different ROIs are color coded corresponding to the associated graph showing recovery kinetics. The signal in the blue ROI was used to subtract the background signal. Signal was bleached in the red ROIs and recovery was monitored in all the ROIs. The analyses here does not include correction for bleaching and is normalized by the initial fluorescence.