Table 1. Scanning parameters for acquiring high resolution Z stacks of mitochondria.

Parameter	Value
*Zoom	2.5-3
Pixel size	1024×1024
Scan speed	7
Scan number for averaging	2
Pinhole	1 airy unit
Laser intensity	1-20%
Detector gain	600

^*High resolution imaging of mitochondria would limit the investigator to scan one cell at a time, or part of a cell or even individual mitochondria. To image a whole cell the zoom factor (digital magnification) will have to be decided based on the size of the cell. A zoom factor of more than 5 is not recommended as this would cause the image to be pixelated but can be used, if absolutely necessary, with adjusting other parameters.