Table 4. Imaging parameters for measuring mitochondrial potential per unit mass.
| Parameter | Individual cell | Individual mitochondria |
|---|---|---|
| Objective | 40× | 63× |
| Zoom | 0.7 | 2-3 |
| Scan speed | 8 | 8 |
| Averaging | 2 | 2 |
| Optical slice | Open | 1μm |
| Z section | -------- | 0.5μm |
| *Laser power | 1-10% | 1-10% |
*
The TMRE imaging of mitochondria at zoom 2 or 3 should be done very cautiously as high laser power could quickly photodamage TMRE loaded mitochondria (Bunting 1992). Therefore for optimum results scanning laser intensity of the both the lasers should be kept as low as possible.