Figure 6. RDD gene therapy and mediators of endothelial functions in psoriatic skin transplanted onto immuno­deficient mice.

(A) Uninvolved skin from psoriasis patients was transplanted onto immuno­deficient Prkdc^scid mice, and the recipient mice were treated as outlined in Methods to induce psoriasis (PN, top row), or psoriatic skin with a full-fledged phenotype (PP, bottom row) was transplanted onto Prkdc^scid mice. The recipient mice were either subjected to control treatment using pVAX-mock or pVAX-RFP or pVAX-RDD. Expression of HIF1α was detected by immuno­histo­chemistry. Scale bar: 50 μm. (B) Expression of angiopoietin-1 (ANG1) was detected by immuno­histo­chemistry in transplants from mice treated as described in A. Scale bar: 50 μm. (C) Expression of VEGF-1 was detected by immuno­histochemistry in transplants from mice treated as described in A. Scale bar: 50 μm. (D) Expression of FLT1 was detected by immuno­histo­chemistry in transplants from mice treated as described in A. Scale bar: 50 μm. (E) Expression of CD1a was detected by immuno­histochemistry in psoriatic transplants from mice treated as described in A. Scale bar: 50 μm.