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Human SOX3 (100 ng) activates hTES-luc (1.6 μg) (10–fold) compared with human SRY (100 ng) (5-fold) in CHO cells. In the presence of added SF1 (20 ng), further transactivation occurs with both SOX3 (25-fold) and SRY (20-fold) (n = 2–3). Error bars represent SD. Unless otherwise indicated, statistical comparisons were made between fold activations of expression vector and control empty vector. ***P < 0.001, **P < 0.01.
