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. 2010 Dec 22;121(1):328–341. doi: 10.1172/JCI42580

Figure 8. Expression of Aldh1a1 and EGFP in the gonadal ridge of XX Tg/+ gonads at 6–17 ts.

Figure 8

Immunohistochemistry and in situ hybridization of serial transverse sections showing EGFP and Aldh1a1 distribution in 6–17 ts embryos. Given the low level of Aldh1a1 protein in the XX Tg/+ GR during these early stages of sex determination, the expression of Aldh1a1 in adjacent sections is included at each stage for comparison. Aldh1a1/Aldh1a1 is first observed at 6 ts (magnified in box inset). Aldh1a1/Aldh1a1 is detected in a diffuse pattern at 12 ts. A rare EGFP-positive cell is found within a population of Aldh1a1-positive cells in the GR at this stage (magnified in box 1). At 17 ts, Aldh1a1/Aldh1a1 becomes more restricted, while the abundance of EGFP within the GR increases (magnified in box 2). Scale bars: 100 μm.