Figure 8. Expression of Aldh1a1 and EGFP in the gonadal ridge of XX Tg/+ gonads at 6–17 ts.
Immunohistochemistry and in situ hybridization of serial transverse sections showing EGFP and Aldh1a1 distribution in 6–17 ts embryos. Given the low level of Aldh1a1 protein in the XX Tg/+ GR during these early stages of sex determination, the expression of Aldh1a1 in adjacent sections is included at each stage for comparison. Aldh1a1/Aldh1a1 is first observed at 6 ts (magnified in box inset). Aldh1a1/Aldh1a1 is detected in a diffuse pattern at 12 ts. A rare EGFP-positive cell is found within a population of Aldh1a1-positive cells in the GR at this stage (magnified in box 1). At 17 ts, Aldh1a1/Aldh1a1 becomes more restricted, while the abundance of EGFP within the GR increases (magnified in box 2). Scale bars: 100 μm.