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. 2010 Dec 1;121(1):86–95. doi: 10.1172/JCI42846

Figure 4. Dysregulation in BA synthesis and transport pathways results in severe cholestasis in DKO mice.

Figure 4

Total RNA (n = 4) for each time point was prepared, and gene expression was analyzed in duplicates using NanoString technology. Genes involved in synthesis of (A) neutral and (D) hydrophobic BA are increased but those involved in acidic BA (B and C) are decreased. (E–G) Aberrant expression of genes involved in BA efflux into bile. (H–J) Genes involved in BA efflux into circulation are increased, whereas (K and L) those involved in BA uptake into liver are decreased. (M–P) Genes involved in BA detoxification are increased. (Q) Cyp7A protein levels are induced dramatically in DKO animals and are quantified in R. Throughout the figure, fold change is shown with respect to WT at 3 weeks. Data are presented as mean ± SEM. *P < 0.05, **P < 0.001 when compared with their respective age-matched WT animals.