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. 2010 Dec 22;121(1):148–160. doi: 10.1172/JCI42874

Figure 3. ATO does not inhibit GLI1 by affecting primary cilia.

Figure 3

(A) Effects of ATO on primary cilia were visualized using confocal fluorescence microscopy on MEFs stained for acetylated tubulin. Arrows indicate cilium. Scale bars: 20 μm. (B) KIF3a WT and knockout cells were transfected with pGL38XGLI and EGFP-GLI1. The Renilla-TK construct was used as a control for normalization. 24 hours later, cells were treated for 24 hours with ATO at 1, 3, and 10 μM. Mean ± SD relative luciferase activity was calculated relative to Renilla activity. Transfection assays were performed in triplicate. (C) Expression of GLI1 and KIF3a were detected by IB.