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. 2010 Dec 22;121(1):132–147. doi: 10.1172/JCI42912

Figure 6. Tumor endothelin contributes to early macrophage infiltration and inflammation in the lung.

Figure 6

(A) ET-1 depletion in UMUC3 cells transduced with shET-1 and NTsh lentiviruses was determined by Western blotting. The burden of UMUC3 cells in the lungs at the indicated times was determined by 12p PCR. Bars represent mean ± SEM of amount of 12p DNA (ng) detected in lungs of 4 animals/group. (B) The number of macrophages infiltrating the lungs (×100 magnification) after tail vein injection of shET-1 and NTsh cells, at the indicated times. Bars represent the mean ± SEM of the number of macrophages (MF), counted as described above. ET-1 and COX-2 activity (C), hIL-6 and hMCP-1 (D), and mIL-6 and mMCP-1 (E) were determined in lungs at the indicated time points. Bars represent mean ± SEM of tissue lysates from 5 animals/group performed in triplicate. *P < 0.05, Student’s t test, comparing UMUC3-NTsh lungs at 24 hours with those at 48 hours; **P < 0.01, Student’s t test, comparing UMUC3-shET-1–injected lungs with UMUC3-NTsh counterparts at the same time point. NC, normal control.