Figure 3.

Northern-blot analysis of transcript accumulation in wild-type and elm1 seedlings. Total RNA was extracted from WT and elm1 seedlings grown in W light or after a shift to D. RNA was extracted from leaf tissue harvested at time points indicated. Approximately 5 μg of total RNA was used in northern-blot analysis. The 27S ribosomal band was used as a loading control and was visualized using ethidium bromide staining after membrane transfer. Gene-specific fragments of Ppc, RbcS2, and Me1 were hybridized to the filters according to the protocols described in “Materials and Methods.”