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Northern-blot analysis of Ppc transcript accumulation in wild-type and elm1 seedlings. Total RNA was extracted from leaf tissue (T), M protoplasts, stressed control (S), and BS cells of plants grown in W light for 12 d and shifted into D, B, R, and FR. Approximately 5 μg of total RNA was used in northern-blot analysis. The 27S ribosomal band is shown as a loading control.
