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. 2010 Dec 1;121(1):113–119. doi: 10.1172/JCI44073

Figure 2. Patients’ sera detected natural form of PIT-1 by immunohistochemistry, and the antibodies were of IgG1 and IgG3 subtypes.

Figure 2

(A) Immunohistochemical analysis revealed that patients’ sera, as well as anti–PIT-1 antibody, recognize human anterior pituitary cells in the normal pituitary tissue. Original magnification, ×600. Preabsorption of the sera with rhPIT-1 substantially reduced the signal, indicating the specificity for PIT-1. (B) The anti–PIT-1 antibody belongs to the IgG1 and IgG3 fractions in case 1 and to the IgG1 fraction in cases 2 and 3 (B and Supplemental Figure 3E).