Figure 4. Tissue and subcellular distribution of SLC1A1.

(A–D) Immunofluorescence of hemagglutinin epitope-tagged WT and mutant cDNAs stably expressed in MDCKII cells (A) SLC1A1 WT, (B) SLC1A1 R445W, and (C) SLC1A1 I395del MDCKII, and (D) empty vector (pcDNA3.1-HA). Scale bar: 10 μm. (E–H) Immunofluorescence of normal adult human kidney, showing SLC1A1 expression in proximal tubules. Kidney sections were stained with an SLC1A1 antibody (red) and the proximal tubule marker LTA (green). Nuclei were stained with DAPI (blue). (E) SLC1A1 expression on the apical membrane of kidney tubules. An example of an S1 segment (arrow) emerging from the glomerulus (g) is indicated. (F) LTA staining identifying the apical membrane of proximal tubules. (G) Colocalization (yellow) of SLC1A1 and LTA on the apical membrane of S1 segments of the proximal tubule (confocal images in E and F were merged). (H) Peptide-blocked control. A glomerulus is indicated. Scale bar: 30 μm.