Figure 1. Leptin signaling in Kiss1 neurons is not required for puberty and fertility.

(A) Product of PCR showing sites of DNA recombination in male and female mice with deletion of LepR from Kiss1 cells compared with a control mouse. Note the deletion of LepR from Kiss1 cells in the hypothalamus and gonads; (B) coexpression of leptin-induced pSTAT3 in Kiss1 neurons of the Arc; (C) quantification of Kiss1 neurons expressing leptin-induced pSTAT3 in the Arc of Kiss1-Cre LepR^flox/flox mice and of LepR^flox/flox littermates. (D–F) Graphs showing no changes in body-weight (D), in time for pregnancy (E), and in fecundity (number of offspring, F) comparing Kiss1-Cre LepR^flox/flox and LepR^flox/flox littermates. *Statistically different from LepR^flox/flox littermates. Data are expressed as mean ± SEM. Scale bar: 200 μm.