Figure 7. Neurons in the PMV coexpress glutamate and LepR and make apparent contacts with Kiss1 neurons and GnRH terminals.

(A) Distribution of vesicular glutamate transporter 2 (vGluT2) mRNA in the PMV of LepR-cre LacZ (reporter) female mice. (B) PMV neurons coexpress vGluT2 mRNA (silver grains, ³⁵S-labeled riboprobe) and β-gal immunoreactivity (β-gal-ir, brown cytoplasm, arrows). (C) Injection site of the anterograde tracer BDA into the PMV. (D) BDA fibers (green) in close apposition to GnRH immunoreactive fibers (red) in the median eminence/mediobasal hypothalamus (arrows). (E) Innervation of the anteroventral periventricular nucleus (AVPV) by PMV neurons; (F) close appositions (arrows) between fibers originating from PMV neurons (in green) and Kisspeptin immunoreactive (Kisspeptin-ir) neurons of the AVPV (in red). Scale bars: 400 μm (A, C, E); 80 μm (B and D); 50 μm (F).