Fig. 4.

Effect of 10,12-CLA dose on mammary gland weight and expression of lipogenic genes. Female FVB mice received 10,12-CLA at doses of 0, 0.1, 0.2, or 0.5% of the diet from day 24 to 49 of age (n = 8 to 10 per treatment). A: mammary glands were collected from mice at 49 days of age and weighed. Each bar represents the mean ± SE of 8 to 10 mice. B: total RNA was extracted and analyzed by real-time PCR for mRNA abundance of sterol regulatory element binding protein-1c (SREBP-1c), fatty acid synthase (FASN), and thyroid hormone responsive spot 14 (S14). Data are normalized to 18S expression. Each bar represents the mean ± SE of 8 mice. ^a,b,c,dBars with different letters are significantly different, P < 0.05.