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. 2010 Sep 15;299(6):R1521–R1528. doi: 10.1152/ajpregu.00445.2010

Fig. 5.

Fig. 5.

Effect of 10,12-CLA dose on gonadal fat weight and expression of lipogenic and inflammatory genes. Female FVB mice received 10,12-CLA at doses of 0, 0.1, 0.2, or 0.5% of the diet from day 24 to 49 of age (n = 8 to 10 per treatment). Gonadal fat was collected at day 49 of age. Total RNA was extracted, and the expression of specific genes was analyzed by real-time PCR. A: mass of gonadal fat. Each bar represents the mean ± SE of 8 to 10 mice. B: expression of SREBP-1c, FASN, and S14. Data were normalized to 18S. Each bar represents the mean ± SE of 6 or 7 mice. C: expression of chemokine MCP-1, the macrophage marker EGF-like module containing EMR1, TNF-α, and IL-6. Data were normalized to 18S expression. Each bar represents the mean ± SE of 5 mice. a,b,cBars with different letters are significantly different, P < 0.05.