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. 2010 Sep 15;299(6):R1592–R1601. doi: 10.1152/ajpregu.00247.2010

Fig. 4.

Fig. 4.

Effects of glucocorticoids on C/EBP-δ mRNA levels and promoter activity. A: gel showing the effects of 24 h of vehicle + green fluorescent protein (GFP (left) and glucocorticoid receptor (GR) + 1 μM dexamethasone (DEX) treatment (right) on C/EBP-δ (top) and GAPDH (bottom) mRNA levels. B: quantification of C/EBP-δ mRNA levels. Twenty four hours of GR + DEX treatment significantly increased C/EBP-δ mRNA levels relative to GFP + vehicle controls. Bars represent means ± SE for 5 independent transfections. *Significantly different from GFP vehicle-treated control, P < 0.05. C: mouse C/EBP-δ promoter activity after 24 h of treatment with GFP cotransfection and vehicle alone (GFP vehicle), GFP cotransfection and 1 μM DEX, GR cotransfection and vehicle alone (GR vehicle), or glucocorticoid receptor cotransfection with 1 μM DEX (GR DEX). GR cotransfection with and without DEX cotreatment significantly increased activity of the mouse C/EBP-δ promoter at 24 h effect on mouse MSTN promoter activity with or without DEX cotreatment at either time point. Bars represent means ± SE for 3 independent transfections with 6 wells/transfection. *Significantly different from GFP vehicle-treated control, P < 0.05. †Significantly different from GFP DEX treated, P < 0.05. ‡Significantly different from GR vehicle treated, P < 0.05.