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. 2003 Dec;133(4):1630–1642. doi: 10.1104/pp.103.029751

Figure 2.

Figure 2.

Phytochrome-dependent germination response. A, Summary of seeds used in the germination experiment. WT, Wild type. B, Germination frequencies of wild-type or various transgenic plant lines overexpressing HFR1 or HFR1-ΔN105 seeds were measured. The seeds were treated with FR light (21 μW cm-2) for 15 min just after imbibition and then transferred to darkness without or with exposure to R light (33 μW cm-2) for 10 min. Seeds were then incubated in darkness for an additional 5 d. Each experiment was performed with at least 150 seeds. Similar results were obtained from three independent experiments. C, Representative plates from the germination experiments described in B. The plates were given a pulse of FR light and kept in darkness. D, Representative plates from the germination experiments described in B. The plates were given a pulse of R light after a pulse of FR light and then kept in further darkness.