Table 1.
Morphometric and plasma characteristics
| Group |
P Value |
|||||
|---|---|---|---|---|---|---|
| Lean |
Obese |
|||||
| Variable | Cycling | OVX | Cycling | OVX | Obesity effect | OVX effect |
| Weight gain,† g/day | 0.51 ± 0.49 | 2.91 ± 0.35 | 0.44 ± 0.30 | 3.01 ± 0.41 | 0.976 | <0.001 |
| Body wt,* g | 308.8 ± 7.4 | 318.1 ± 6.1 | 380.3 ± 14.0 | 390.4 ± 9.3 | <0.001 | 0.342 |
| FFM,* g | 237.6 ± 5.1 | 246.7 ± 3.9 | 268.9 ± 9.2 | 275.7 ± 6.1 | <0.001 | 0.240 |
| FM* | ||||||
| g | 71.2 ± 5.0 | 71.5 ± 4.9 | 111.4 ± 6.0 | 114.8 ± 6.2 | <0.001 | 0.753 |
| % | 22.9 ± 1.3 | 22.3 ± 1.2 | 29.2 ± 0.9 | 29.2 ± 1.2 | <0.001 | 0.796 |
| Glucose,† mM | 9.84 ± 0.5 | 12.95 ± 0.8 | 11.05 ± 0.7 | 14.02 ± 0.7 | 0.207 | <0.001 |
| Cholesterol, mM | 2.06 ± 0.3 | 2.11 ± 0.4 | 1.88 ± 0.3 | 2.34 ± 0.3 | 0.953 | 0.459 |
| TGs,* mM | 0.93 ± 0.1 | 1.08 ± 0.2 | 1.53 ± 0.3 | 2.43 ± 0.6 | 0.008 | 0.140 |
| NEFAs,† μM | 714.0 ± 79.6 | 433.4 ± 58.0 | 650.8 ± 55.3 | 602.8 ± 66.8 | 0.546 | 0.048 |
| Estradiol,† pM | 167.2 ± 18.3 | 52.7 ± 8.6 | 187.6 ± 19.3 | 49.5 ± 5.3 | 0.886 | <0.001 |
Values are means ± SE. For rate of weight gain, all animals (n = 20–25 per group) were included in the analysis; for plasma and some morphometric measurements, a representative subset of animals (n = 10–14 per group) was used. FFM, fat-free mass; FM, fat mass; TGs, triglycerides; NEFAs, nonesterified fatty acids. Body composition was determined through a combination of dual-energy X-ray absorptiometry and quantitative magnetic resonance imaging. Plasma was collected on diestrus 2 (cycling animals) or at the time of death [ovariectomized (OVX) animals], and cholesterol, TGs, NEFAs, glucose, and estradiol were measured using standard assays. Data were examined by ANOVA, with significance set at P < 0.05:
obesity effect;
OVX effect.